The increasing demand for new pharmaceutical products has encouraged manufacturers to seek new bioengineering strategies for the development of competitive biotechnological processes for the recovery and purification of proteins. The new trend in the development of recovery systems, exploits novel approaches of bioprocess integration and intensification. In this paper, some of the achievements of the practical implementation of such bioengineering strategies for the development of biotechnological processes using defined experimental vehicles, are addressed. An arbitrary selection of bio-processes using aqueous two-phase systems (ATPS) and expanded bed adsorption (EBA) to implement these novel approaches is reported with the aim of establishing the benefits of such strategies. The successful cases of intracellular protein recovery from baker's yeast, the development of a highly simplified process to c-phycocyanin recovery from Spirulina maxima and the radical approach for recovering inclusion body proteins are discussed. It is proposed that the trend of the practical application of the novel bioengineering strategies for the recovery of protein products will address the current process requirements. Such trend will give impetus to the development of bioseparation systems, and will draw attention from industries needing to develop new, and improve existing, commercial bioprocesses.