This article presents the theoretical support and empirical validation of an instrument designed to measure learning styles. The instrument considers five dimensions (hence the term pentavalent): chronobiological, sociological, level of dependence on others, sensory preferences in general and sensory preferences in the use of technology. The instrument had 108 items measurable with the Likert scale, grouped into 18 scales. The results show descriptive statistics data of the sample that was taken as a frame of reference for the establishment of norms. It also includes an analysis of the reliability (internal consistency), validity, and item analysis to determine the psychometric characteristics of the instrument. The Pentavalent inventory for measuring learning styles is proposed as a useful tool to adapt the design of learning environments based on students' individual preferences.