Insulator-based dielectrophoresis of protein particles using direct current electric fields uri icon


  • Dielectrophoresis is a method of rapid response with sufficient selectivity for manipulation and separation of bioparticles, such as: microorganisms and biomolecules. Due to the great importance of proteins in the biotechnological and pharmaceutical processes, the present study demonstrates the potential of the insulator-based dielectrophoresis (iDEP) and DC electric fields to manipulate and concentrate protein solutions, using bovine serum albumin as a model. Samples containing fluorescently labeled bovine serum albumin protein were manipulated inside a microchannel made from glass that contained an array of cylindrical insulating structures. DC electric fields were applied and the dielectrophoretic response of the particles was observed. It was shown that the magnitude of the applied electric field and the conductivity and pH of the suspending medium have a strong effect on the dielectrophoretic response of the protein particles.

Publication date

  • December 1, 2008