Effect of high hydrostatic pressure on mycelial development, spore viability and enzyme activity of Penicillium Roqueforti
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This study investigated the effect of high hydrostatic pressure treatments on mycelial development, spore viability, and total proteolytic and lipolytic activity of Penicillium roqueforti PV-LYO 10 D. Fungus growing in liquid medium was pressure-treated at 300, 400, and 500. MPa for 10. min at 20. °C following seven days of incubation at 25. °C and analyzed periodically up to day 9 after treatments to evaluate the effect on fungal growth. Mycelial mass of P. roqueforti was significantly affected at all pressure treatments evaluated, being 15.48%, 22.28%, 30.03%, and 12.53% lower than controls on day 1, 3, 6, and 9 after 300. MPa treatment, respectively. In a similar way, at 400 and 500. MPa, mycelial mass was 31.08% and 60.34% lower than controls one day after treatments and 49.74% and 80.85% lower on day 9, respectively. The viability of P. roqueforti spores decreased by 36.53% at 300. MPa, and complete inactivation took place at. ¿. 400. MPa from an initial count of 7 log cfu/mL. Total proteolytic activity was not significantly affected at 300. MPa but was reduced by 18.22% at 400. MPa and by 43.18% at 500. MPa. Total lipolytic activity also decreased as the intensity of the pressure treatments increased. 21.69%, 39.12%, and 56.26% activity reductions were observed when treatments of 300, 400 and 500. MPa were applied, respectively. The results from this study show that pressure treatments are able to control growth, inactivate spores, and alter enzyme activity of P. roqueforti, which could be of interest in extending the shelf-life of blue-veined cheeses and other food products. © 2013 Elsevier B.V.