AUTOMATIC DETERMINATION OF TRAPPING VOLTAGE IN DIRECT-CURRENT INSULATOR-BASED ELECTROKINETIC DEVICES

© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.Accurately separating the fluorescence intensity of fluorescent-labeled particles from background (BG) levels becomes a challenge when analyzing low concentration samples. To address this challenge in particle trapping experiments, we developed a technique to standardize the quantification of the collected particles within direct-current insulator-based electrokinetic (DC-iEK) microfluidic chips. This automated system accurately predicts the trapping values reported in literature within <5% error. We then fabricated and tested a DC-iEK device that reduces the trapping voltage when compared to the conventionally used circular post geometry. The technique herein can be easily adapted for other insulator-based platforms that rely on fluorescence detection.

AUTOMATIC DETERMINATION OF TRAPPING VOLTAGE IN DIRECT-CURRENT INSULATOR-BASED ELECTROKINETIC DEVICES Academic Article in Scopus