Case studies in the application of aqueous two-phase processes for the recovery of high value biological products
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Mammalian, microbial (bacterial and yeast), and plant cells are effective expression systems used commercially to produce mass quantities of biological, pharmaceutical, or chemical products of interest. However, there is the need to establish selective and scalable methods of product recovery that integrate effectively with upstream cell cultures to rapidly yield products in a state suitable for validation operations. The current state of the art purification and recovery methods utilize well-established multi-step processes (e.g. product release, solid-liquid, concentration and chromatography steps) that usually result in low yield and high process cost. In this key note address a series of case studies were presented where an aqueous two-phase system (ATPS) extraction, an existing bioengineering strategy, was used to alleviate many of the previously mentioned existing process constraints. Specific case studies utilizing ATPS were presented for the purification of Rotavirus-like particles from insect cells, colorant proteins (i.e. C-Phycocyanin and B-Phycoerythrin) from microbial origin, human granulocyte-colony stimulating factor (hG-CSF) from alfalfa, fractionation of PEGylated proteins, and stem cells. Conclusions will be drawn concerning the use of ATPS in downstream processes that can greatly simplify the current way in which bioproducts are recovered. © 2013 American Chemical Society.