abstract
- © 2015 The Authors.In this work, the biocatalytic ability of laccases from filtered culture supernatant of Pycnoporus sanguineus was evaluated without mediators and under mild reaction conditions. This 100 U L -1 laccase cocktail removed 50% Diclofenac, 97% ß-Naphthol and 71% 2,4 Dichlorophenol within 8 h of reaction and 78% for 5,7-Diiodo-8-hydroxyquinoline within 3.5 h; at initial concentrations of 10 mg L-1 and at 25 °C. Furthermore, this enzyme cocktail also removed in excess of 53% all tested compounds in a real groundwater sample from northwestern Mexico. In comparison with purified laccases, the use of cocktail offers operational advantages since additional purification steps can be avoided.