TMB vs ABTS: comparison of multi-enzyme-based approaches for the colorimetric quantification of salivary glucose Academic Article in Scopus uri icon

abstract

  • © 2022 Society of Chemical Industry (SCI).BACKGROUND: Diabetes is one of the fastest-growing health problems of recent times. Its diagnosis, monitoring and control are carried out mainly by glucose quantification in blood samples, which can be a traumatic and painful procedure for many patients. Saliva is an emerging diagnostic biofluid for minimally invasive disease diagnosis. Its collection can be performed through a simple, noninvasive and painless procedure that does not require qualified personnel. This project aimed to develop, standardize and compare two enzyme-based approaches (TMB and ABTS) for the colorimetric quantification of salivary glucose. RESULTS: The obtained results with both dyes revealed the suitability of these systems for glucose quantification under buffer conditions, displaying excellent linearity (R2 > 0.99) and precision (CV < 1%), and high glucose specificity. Furthermore, the TMB system was able to quantify not only glucose in human saliva samples, showing a low read-out variability (CV < 3%), but also glucose changes (16 and 50 ¿mol L¿1) with low variability (CV < 4.2%) and excellent recovery (96¿103%), which is important in clinical diagnosis. CONCLUSION: Altogether these results demonstrated the capacity of the approaches in quantifying glucose either in buffer (using both TMB ans ABTS) or in human saliva samples (using TMB). They offer a painless, simple, efficient, and relatively inexpensive analytical method with enormous potential for the diagnosis and monitoring of diabetes using a small amount of saliva. © 2022 Society of Chemical Industry (SCI).

publication date

  • January 1, 2022