The Potential of Rutin and Curcumin in Inducing Differentiation of Mouse C3H10 Cells Towards a Neuronal-Like Lineage Academic Article in Scopus uri icon

abstract

  • The role of curcumin and rutin individually and in combination for inducing differentiation in the mouse embryonic C3H10 cell line, which exhibits fibroblast morphology and displays mesenchymal stem cell-like properties, towards a neuronal lineage was examined. The neuronal phenotype and the neuronal-specific gene expressions like Nestin, ChAT, NeuN, MAP2, Eno2, Neurod1, and Ntf3 were assessed. To evaluate the binding affinity of the neuronal-specific proteins (Nestin, ChAT, NeuN, MAP2, Eno2, Neurod1, and Ntf3) with the natural compounds, molecular docking was performed. Neuron-specific cell body was studied by toluidine blue stain, neutral red was used for Nissl body staining, and periodic acid-Schiff¿s glycoprotein staining demonstrated mouse C3H10 cells potential to differentiate into cells with neuronal characteristics upon treatment with curcumin and rutin, providing insights into their capability for promoting neuronal-like differentiation. Upregulation of neuronal genes, such as ChAT, NeuN, and MAP2, after curcumin and rutin treatment and its in silico affinity for neuronal proteins, demonstrates the possible effectiveness of curcumin and rutin in inducing neuronal cell-like differentiation. All experimental groups showed neuronal differentiation, although the combination of curcumin and rutin treatment significantly increased the maximum neurite outgrowth per neuron and expression of neuronal-specific genes. This study found that selective dosages of curcumin and rutin could increase the C3H10 cells differentiation into neuronal-like cells. Graphical Abstract: (Figure presented.) © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2024.

publication date

  • January 1, 2024