Advancement of green process through microwave-assisted extraction of bioactive metabolites from Arthrospira Platensis and bioactivity evaluation
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© 2016Bioactivity and functional properties of cyanobacterial extract mostly depends on process of extraction, temperature and solvent used (polar or non-polar). To evaluate these parameters a design of experiment (DOE; using a 2k design) was performed with Arthrospira platensis. Extraction process was optimized through microwave-assisted extraction considering solvent ratio, temperature and time of extraction with polar (PS) and non-polar (NPS). Maximum extract yield obtained was 4.32 ± 0.25% and 5.26 ± 0.11% (w/w) respectively for PS and NPS. Maximum content of bioactive metabolites in PS extracts were thiamine (846.57 ± 14.12 ¿g/g), riboflavin (101.09 ± 1.63 ¿g/g), C-phycocyanin (2.28 ± 0.10 ¿g/g) and A-phycocyanin (4.11 ± 0.03 ¿g/g), while for NPS extracts were ¿-tocopherol (37.86 ± 0.78 ¿g/g), ß-carotene (123.64 ± 1.45 ¿g/g) and 19.44 ± 0.21 mg/g of fatty acids. A. platensis PS extracts showed high antimicrobial activity and PS extracts had antioxidant activity of 0.79 ± 0.12 ¿molTE/g for FRAP assay, while for NPS extracts 1.03 ± 0.08 ¿mol ¿-TE/g for FRAP assay.