Partial recovery of mrjp1 protein expressed in pichia pastoris using chromatographic techniques Recuperación parcial de la proteína mrjp1 expresada en pichia pastoris utilizando técnicas cromatográficas Academic Article in Scopus uri icon

abstract

  • © 2021, Universidad Autonoma Metropolitana. All rights reserved.Major Royal Jelly Protein 1 (MRJP1) is the main protein in the complex mixture royal jelly, which is the only nutrient source for queen bees. The protein promotes increased lifespan, body size and fertility. Recombinant production of MRJP1 represents an alternative to direct extraction from royal jelly. Production in Pichia pastoris results in high density biomass, and a complex supernatant containing high amount of impurities. Various chromatography methods have been applied to recover and/or purify MRJP1. In this work, exploiting the physicochemical properties of MRJP1, reverse phase chromatography (RPC), size exclusion chromatography (SEC) and ion-exchange chromatography (IEX) were investigated as alternative methods to recover MRJP1 directly from supernatant. ACl techniques showed a 57-kDa band in SDS-PAGE analysis, corresponding to the size of recombinant MRJP1, with contaminants attributed to culture media. However, the chromatogram from SEC coupled to IEX shown a single peak suggesting it may be a good protocol to recover recombinant MRJP1 from P partoris suprrnatant. This is the first report about partial recovery of recombinant MRJP1 produced in Pichia pastoris utilizing IEX without the use of histidine tags.

publication date

  • January 1, 2021