abstract
- In recent years, there has been a growing interest in plant-based diets, particularly legumes, as a sustainable and healthy dietary choice. This study breaks new ground by investigating the effects of simulated gastrointestinal digestion on green (Leucaena leucocephala) and pigmented (Leucaena esculenta) guaje proteins. We evaluated the antioxidant and immunomodulatory properties of ultrafiltered fractions resulting from digestion in a macrophage model. Both fractions showed promising potential as radical scavengers. The fraction <5 kDa from pigmented guaje, even at the lowest doses tested, significantly (p < 0.05) inhibited the release of pro-inflammatory cytokines TNF-¿ and IL-6, and demonstrated an immunomodulatory effect by reducing the levels of ROS and NO. These findings suggest that green and pigmented guaje could be a valuable source of bioactive peptides, potentially used as a coadjutant for treating and preventing oxidative stress and inflammation-associated non-communicable diseases through the utilization of underutilized legumes. © 2024 Elsevier Ltd