Development, characterization, and comparison of chitosan microparticles as a carrier system for black bean protein hydrolysates with antioxidant capacity Academic Article in Scopus uri icon

abstract

  • Abstract: Peptides in black bean protein hydrolysates (BPHs) exert antioxidant capacity. However, peptides are prone to degradation during processing and digestion. Chitosan (Ch) can protect them and provide a delayed release. This work develops and compares two drying methods producing porous structured Ch microparticles (MPs) as carriers for antioxidant BPH. Ch gels were obtained by ionic gelation and dried by supercritical CO2 solvent displacement or fast-freeze-drying methods. The resulting aerogels and fast-freeze-dried MPs were structurally characterized, and their swelling and release profiles were obtained at pH 1.2 and 7.4. The antioxidant capacity of systems was determined by 2,2¿-azino-bis(3-ethyl-benzthiazoline-6-sulphonic acid) (ABTS) and superoxide radical assays. The results showed BPH-Ch best complexation conditions occurring at a pH of 4.5 and a 4:1 BPH/Ch ratio. The particle size of the complex was 1047.6 nm, and the entrapment efficiency and loading capacity were 28.2% and 54.3%, respectively. At pH 1.2 and 7.4, the release rate of BPH was lower in aerogel than in fast-freeze-dried MPs. Besides, entrapment BPH in Ch significantly reduced the ABTS antioxidant activity IC50 from 35.1 µM Trolox equivalents (TE)/mg to 250.7 and 406.2 µM TE/mg for Ch fast-freeze-dried and aerogels, respectively. Superoxide radical inhibition IC50 ranged from 74.6 to 92.9 mM ascorbic acid equivalents/mg in the different samples. BPH-loaded aerogels presented lower specific surface area (94.7 vs. 138.6 m2/g, p < 0.05) and higher average pore size (26.4 vs. 19.8 nm) than Ch aerogels. Ch aerogel is a promising carrier for delaying the release of common bean antioxidant peptides useful for developing functional foods. Practical Application: This novel system could act as an ingredient to incorporate antioxidant compounds in different formats to develop delayed-release nutraceuticals and functional foods, such as bakery, dairy products, or beverages. Along, antioxidant peptide-loaded aerogels could be used as a slow-release system for compounds acting as natural preserving antioxidants for food applications such as raw meat products or high-fat foods. © 2024 Institute of Food Technologists.

publication date

  • January 1, 2024