Aqueous two-phase systems Book in Scopus uri icon


  • © 2019 Elsevier B.V. All rights reserved.Aqueous two-phase system (ATPS) is a liquid-liquid separation technique that has shown great potential for the extraction, recovery, and purification of a great variety of biological compounds. The main advantages of this technique over other separation methodologies for proteins and other compounds include scaling-up feasibility, process integration capability, biocompatibility, and, most importantly, continuous operation. In this article, a general overview regarding the basis and applications of ATPS is presented. The general theory concerning the formation of ATPS and the parameters with significant effect on the partition behavior of biological products are presented. Different strategies for the integration of bioprocesses using ATPS as well as the bioaffinity partition of proteins and other molecules are discussed. Subsequently, the practical application of ATPS-based strategies for the downstream processing of biological products including proteins and nucleic acids, bionanoparticles (virus, virus-like particles, and nanostructures), biomass (cells, cell debris, and organelles), and low-molecular-weight compounds (metabolites and phytochemicals) is presented, and other ATPS-based applications and future trends are briefly discussed.

publication date

  • January 1, 2019