Evaluation of the detection results obtained from ELISA Chapter in Scopus uri icon

Abstract

  • © The Author(s) 2018. This chapter presents the most commons errors that typically occur when performing ELISA. Following every error, the chapter points out the possible reasons behind such errors and possible methods to overcome the problems. The chapter also describes key important parameters in assay evaluation including sensitivity, specificity, accuracy and limit of detection (LOD). Offering methods for calculation of the evaluation parameters, the chapter also provides insights and considerations for assessing the reliability of the assay. Such analysis is essential to upgrade a newly developed assay from the analytical relevance to the clinical application. Finally, the chapter provides information in regard to the measurable units in ELISA assay.

Publication date

  • January 1, 2018