Effect of high CO2concentrations on Desmodesmus abundans RSM lipidome Academic Article in Scopus uri icon

abstract

  • © 2022 The Authors.Lipids are of interest as potential byproducts of CO2 mitigation systems. We characterize the effect of different CO2 concentrations in air (0.04 % pure air, 25 % and 50 % v/v CO2/air) on Desmodesmus abundans RSM lipidome, adapted for eight years to these conditions. Batch cultures grew similarly but more biomass was generated at high CO2 (1.2- to 1.9-fold increase). Lipidome analysis at late exponential, and early and late stationary phase revealed 100 putative lipid features with significant changes grouped into five distinct clusters. Clusters 1 and 2 characterized by glycerolipids (GL, 66-100 %) and clusters from 3 to 5 as glycerophospholipids (GP, 76-100 %). Under air (0.04 % CO2), most features of clusters 1, 2, and 5 were nearly constant through time, but under high CO2, lipid profiles seemed to be determined by dCO2 availability. At dCO2 depletion, GL probably acts as a precursor of GP. During stationary phase, distinct cell maintenance strategies seemed to occur between cultures as lipids or starch were consumed at 25 % and 50 % CO2 to generate GP or GL, respectively. Also, under high CO2, both cultures exhibited an increase in pigments but only under 25 % CO2 a 1.6-fold increase in lipids was observed, which were later consumed. Results suggest different responses to CO2 among cultures; however, at this moment, it is not possible to elucidate whether differences are caused by CO2 treatment or long-term acclimation, or both. Studies should evaluate selected cultures under particular growth conditions to generate specific lipid byproducts for application.

publication date

  • November 1, 2022